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|An analysis of the rate of relaxin may be performed to determine if the dog is pregnant.|
|Development of breast between 25 and 30 days. A loss of hair of udder is often observed at the same time .|
A vaginal discharge occurs after approximately one month.
At this time, loss of appetite may occur.
|Note: at this stage ultrasound does not count the number of puppies.|
|The calcified skeleton to the 45th day. At D +50 , a radio allows to know the number of littermates (or estimate in the case of a large litter).|
|To do if possible every day at the same time to calibrate the average temperature of the dog.|
|The udders begin to produce milk. The uterus contracts slightly.|
|A translucent liquid flows from the vuvle ( melting mucus plug ). 12-24 hours before the onset of labor: the temperature drops sharply, then increased.|
|Dark green or greenish losses indicate the separation of the placenta. The dog begins to push to expel her first puppy.|